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Gradient parameters used for the analysis of cyanotoxins using UHPLC-HESI-HRMS, where A is HPLC water with 0.1% formic acid, B is acetonitrile with 0.1% formic acid, and C is methanol with 0.1% formic acid.
| MCs and ANA-a | CYN, STX and BMAA | |||||
|---|---|---|---|---|---|---|
| Time (min) | A (%) | B (%) | C (%) | Time (min) | A (%) | B (%) |
| 0.00 | 75 | 25 | 0 | 0.00 | 70 | 30 |
| 0.35 | 45 | 55 | 0 | 2.00 | 10 | 90 |
| 1.15 | 45 | 55 | 0 | 4.00 | 0 | 100 |
| 1.16 | 55 | 0 | 45 | 6.00 | 0 | 100 |
| 2.95 | 55 | 0 | 45 | 6.01 | 70 | 30 |
| 4.51 | 5 | 0 | 95 | 10.00 | 70 | 30 |
| 4.66 | 75 | 25 | 0 | |||
| 7.00 | 75 | 25 | 0 | |||
