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Loxosceles venom and SMase D induce the secretion of gelatinases in HK-2 cells. HK-2 cell culture supernatants collected after 24 (A), 48 (B) and 72 h (C) of treatment with Loxosceles venom (V) or SMase D (S) were run on gelatin containing 10% SDS-PAGE gels under non-reducing conditions. Control supernatants (B) were recovered at the same incubation periods from cells incubated with medium plus Phosphate-buffered saline—PBS (B).
