Figure 3.

Tetracycline inhibits Loxosceles venom and SMase D induced cell death in HK-2 cells. HK-2 cell cultures (5 × 10⁴ cells) were incubated with increasing concentrations of tetracycline and cell viability was assessed at 24, 48 and 72 h by the MTT method (A); HK-2 cell cultures (5 × 10⁴ cells) were incubated with venom (V) (B) or SMase D (S) (C) from L. intermedia (5 µg/200 µL total volume), and treated or not with tetracycline (T) [20 µg/mL]. Analyses were performed after 48 h of incubation. All assays were performed in duplicate. Results are representative of three independent experiments and expressed as the mean of duplicates ± standard deviation. Significant differences (*) p < 0.05.