Figure 6.

Histological analysis of the renal lesions induced by Loxosceles venom after tetracycline treatment. BALB/c mice were injected with 4.8 µg of venom (V) and, after 30 min, treated three times every 4 h with 200 µL of tetracycline (T) (5 mg/mL). Control animals were injected with Phosphate-buffered saline—PBS (B). After 24 h, the animals were euthanized and the kidneys collected for histological analysis. (A) Photos correspond to the sections of the kidney stained by H/E. Bars of 2000 μm, 1000 μm and 100 μm for lines I, II and III, respectively. Arrow shows the medullary region; Estimation of glomerular injuries (B) and interstitial changes (C) of the histological samples. Results are representative of two independent experiments and expressed as the mean of triplicates ± standard deviation. Significant differences (*) p < 0.05.