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. 2017 Mar 17;6(1):8. doi: 10.3390/cells6010008

Figure 2.

Figure 2

Figure 2

Hypoxia induces KDM2A protein but has little effect on KDM2B protein levels. (A) U2OS and HeLa cells were exposed to 1% O2 for the depicted times prior to cell lysis. Western blot was performed for the levels of the indicated proteins. Nonspecific band is labelled ns; (B) HeLa and U2OS were grown on coverslips and exposed to 1% O2 for the times depicted prior to fixation with methanol. Cells were stained with anti-KDM2B, HIF-1α antibodies, and DAPI to mark chromatin. Scale bar represents 10 µm. Images were acquired using a Deltavision microscope, and deconvolved and analysed using Omero software. Pixel intensities were quantified in Omero using the region of interest (ROI) tool. Graph depicts mean and standard deviation (SD) of a minimum of 100 cells per condition. Student t-test was performed and p value calculated. * p < 0.05, *** p < 0.001.