Table 1.
Description of the 97 putative protein kinases (PKs) identified in the kinome of Sarcocystis neurona. The putative PKs could be classified into eight groups. The amino acid coordinates of the conserved PK domains in the protein sequences and the PK homologies to other apicomplexan PKs are shown in Columns 7–12.
| Description of the Putative Protein Kinases (PKs) in the Genome of S. neurona | Description of Protein Kinase (PK) Homologies (BLASTp) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Protein ID a | Sequence Annotations; Description b | Family; (Subfamily) c | Length (aa) | pI | MW (kDa) | PK Domain Coordinates | Sequence Name; (Apicomplexan) | Bit Score | E-Value | Identity (%) | Accession Number |
| 1. Kinase Group AGC (Protein kinases A (PKA), G (PKG) and C (PKC) families) | |||||||||||
| SRCN_1312 | AGC kinase | 3-phosphoinositide dependent PK-1 (PDK1) | 903 | 5.52 | 101.13 | 137–481 | PDPK; (T. gondii RUB) | 417 | 6.00 × 10−135 | 59 | KFG61374.1 |
| SRCN_3339 | AGC kinase | PKA | 1428 | 8.85 | 147.52 | 1102–1417 | Putative AGC kinase; (N. caninum L) | 611 | 0.0 | 81 | CEL65574.1 |
| SRCN_4249 | AGC kinase | Nuclear dbf2-related (NDR) | 152 | 8.91 | 17.47 | 6–141 | Putative AGC kinase; (N. caninum L) | 219 | 2.00 × 10−68 | 76 | XP_003883757.1 |
| SRCN_4518 | PK G AGC kinase family member PKG | Ciliate-E2 | 425 | 5.83 | 48.78 | 97–399 | AGC kinase TgPKG1; (T. gondii ME49) | 830 | 0.0 | 92 | EPR61116.1 |
| SRCN_3990 | cAMP-dependent kinase | CAMKL; (MELK) | 1907 | 9.55 | 217.55 | 782–1634 | cAMP-dependent protein kinase (T. gondii VEG) | 75.9 | 1 × 10−12 | 30 | ESS31194.1 |
| SRCN_5165 | cAMP-dependent PK, catalytic chain | PKA | 343 | 8.99 | 39.36 | 20–338 | AGC kinase; (T. gondii ARI) | 425 | 3.00 × 10−150 | 90 | KYF43224.1 |
| SRCN_4913 | Putative PK | PKD | 2330 | 6.42 | 244.64 | 1093–1737 | Putative PK; (E. tenella) | 107 | 9.00 × 10−22 | 60 | XP_013228294.1 |
| SRCN_5430 | AGC kinase | Ribosomal protein S6 Kinases (RSK; (p70)) | 1378 | 5.54 | 139.28 | 824–1344 | AGC kinase; (T. gondii MAS) | 223 | 3.00 × 10−60 | 59 | KFH07588.1 |
| SRCN_5610 | cAMP-dependent PK, catalytic chain | PKA | 333 | 9.00 | 37.96 | 12–318 | cAMP-dependent PK, catalytic subunit; (T. gondii ME49) | 641 | 0.0 | 92 | XP_002366464.1 |
| 2. Kinase Group calcium (Ca2+)-/calmodulin-regulated kinases (CAMK) | |||||||||||
| SRCN_1071 | Ca2+-dependent kinase | CAMK1 | 1495 | 9.47 | 152.66 | 1085–1401 | Putative PK; (T. gondii VEG) | 188 | 1.00 × 10−47 | 67 | ESS31884.1 |
| SRCN_2032 | Putative PK | Ciliate-C1 | 297 | 6.22 | 33.26 | 15–297 | PK; (H. hammondi) | 424 | 1.00 × 10−146 | 68 | XP_008882026.1 |
| SRCN_2165 | Ca2+-dependent kinase CDPK2B | CDPK | 692 | 7.33 | 75.65 | 101–401 | Ca2+-dependent PK CDPK2A; (T. gondii ARI) | 674 | 0.0 | 90 | KYF44522.1 |
| SRCN_2257 | Histone kinase | CAMKL; (AMP-activated protein kinase (AMPK)) | 1800 | 8.98 | 187.71 | 1159–1448 | Putative CAM kinase, SNF1 family; (E. acervulina) | 377 | 7.00 × 10−105 | 64 | XP_013252246.1 |
| SRCN_2544 | CAM SNF1 AMK1 family | CAMKL;(AMPK-regulated kinase novel kinase (NUAK)) | 333 | 5.71 | 37.85 | 62–333 | CAM kinase, SNF1/AMK1 family ToxPK1; (N. caninum L) | 480 | 9.00 × 10−167 | 79 | XP_003882065.1 |
| SRCN_2937 | Ca2+-signaling kinase MARK | CAMKL; (microtubule affinity regulating kinase (MARK)) | 278 | 9.62 | 31.29 | 1–250 | Putative Ca2+ signaling PK MARK; (T. gondii GT1) | 356 | 5.00 × 10−124 | 72 | EPR59053.1 |
| SRCN_3011 | Calmodulin-dependent PK (CAM) CDPK6 | CDPK | 1435 | 9.25 | 154.44 | 1238–1435 | Cdpk kinase domain; (T. gondii) | 179 | 6.00 × 10−49 | 75 | 3IS5_A |
| SRCN_3314 | A Chain crystal Structure of TgCDPK1 with inhibitor bound | CDPK1 | 519 | 5.99 | 58.89 | 37–335 | Calmodulin-domain PK 1; (T. gondii) | 536 | 0.0 | 97 | 3MA6_A |
| SRCN_3583 | Ca2+-dependent kinase CDPK5 | CDPK | 454 | 6.09 | 50.38 | 35–308 | Ca2+-dependent PK CDPK5; (T. gondii ARI) | 776 | 0.0 | 89 | KYF43137.1 |
| SRCN_3701 | Ca2+-dependent kinase CDPK3 | CDPK | 560 | 5.91 | 62.07 | 77–362 | Ca2+-dependent Kinase; (T. gondii) | 493 | 1.00 × 10−172 | 87 | 3DXN_A |
| SRCN_4076 | CAM CDPK family | CDPK | 1701 | 5.93 | 181.42 | 1079–1663 | CAM kinase, CDPK family; (H. hammondi) | 239 | 2.00 × 10−62 | 69 | XP_008884897.1 |
| SRCN_4093 | PK | CAMKL; (AMP-activated protein kinase (AMPK)) | 1155 | 9.02 | 118.33 | 1–261 | Putative atypical MEK-related kinase; (N. caninum L) | 222 | 1.00 × 10−59 | 70 | XP_003880869.1 |
| SRCN_4390 | Ca2+-dependent kinase CDPK2 | CDPK | 790 | 6.24 | 85.47 | 280–556 | Ca2+-dependent PK, related; (N. caninum L) | 1130 | 0.0 | 74 | XP_003884321.1 |
| SRCN_4815 | Histone kinase (partial) | CAMKL; (AMP-activated protein kinase (AMPK)) | 711 | 6.53 | 75.98 | 1–314 | SNF1-related PK catalytic-α KIN10, 5 AMP-activated PK; (N. caninum L) | 568 | 0.0 | 48 | CEL67550.1 |
| SRCN_5227 | CAM CDPK CDPK8-like | CDPK | 2748 | 8.98 | 282.12 | 796–885 | Putative CAM kinase, CDPK family; (N. caninum L) | 117 | 3.00 × 10−24 | 59 | XP_003881901.1 |
| SRCN_5410 | Calmodulin-dependent PK (CAM-SNF1 family) | CAMK1 | 467 | 8.94 | 52.04 | 168–446 | CAM kinase, SNF1 family; (H. hammondi) | 431 | 5.00 × 10−134 | 78 | XP_008883430.1 |
| SRCN_5812 | Ca2+-dependent kinase CDPK9 | CDPK | 760 | 8.37 | 84.23 | 254–573 | Ca2+-dependent PK CDPK9; (H. hammondi) | 1139 | 0.0 | 81 | XP_008889286.1 |
| SRCN_5948 | Ca2+-dependent kinase CDPK8 | CDPK | 3298 | 7.11 | 345.85 | 208–860 | EF-hand domain-containing protein; (T. gondii ME49) | 114 | 1.00 × 10−23 | 58 | XP_002368547.1 |
| SRCN_6597 | Ca2+ dependent kinase CDPK7 | CAMK1 | 1374 | 9.09 | 138.28 | 365–623 | PK-PH domain-containing protein; (T. gondii ME49) | 813 | 0.0 | 80 | XP_002366487.1 |
| SRCN_6606 | Ca2+-dependent kinase CDPK4 | CDPK | 1632 | 9.42 | 170.93 | 813–1236 | Ca2+-dependent PK; (T. gondii) | 731 | 0.0 | 58 | CAD32376.2 |
| 3. Kinase Group casein kinase 1 (cell kinase 1) | |||||||||||
| SRCN_3445 | Casein kinase I | CK1-D | 323 | 9.34 | 37.65 | 6–290 | Casein kinase 1; (T. gondii ME49) | 603 | 0.0 | 94 | XP_002366683.1 |
| SRCN_4587 | Casein kinase I | CK1-D | 137 | 7.78 | 15.94 | 1–137 | Casein kinase I; (H. hammondi) | 226 | 3.00 × 10−71 | 81 | XP_008883809.1 |
| SRCN_4645 | Casein kinase I | CK1-D | 229 | 9.51 | 25.66 | 38–229 | Casein kinase I; (T. gondii GAB2-2007-GAL-DOM2) | 259 | 1.00 × 10−86 | 79 | KFG42638.1 |
| 4. Kinase Group CMGC (including cyclin-dependent kinases, mitogen-activated PKs, glycogen synthase kinases and CDK-like kinases) | |||||||||||
| SRCN_1104 | Cyclin-dependent kinase family 5 | Ca2+-dependent PK-L (CDKL) | 372 | 9.23 | 42.71 | 1–318 | Cyclin-dependent kinase family 5 protein; (H. hammondi) | 490 | 2.00 × 10−173 | 76 | XP_008884207.1 |
| SRCN_1236 | Cell-cycle-associated kinase (SRPK) | Serine-arginine rich PK (SRPK) | 2911 | 5.37 | 302.90 | 713–1837 | PK; (T. gondii ME49) | 476 | 9.00 × 10−141 | 76 | XP_002369401.1 |
| SRCN_1479 | CMGC Lammer | CLK | 748 | 10.23 | 79.09 | 485–748 | Cell-cycle-associated PK CLK; (T. gondii FOU) | 288 | 2.00 × 10−81 | 74 | KFG33061.1 |
| SRCN_1611 | CMGC Dual-specificity tyrosine-regulated kinase (DYRK) | DYRK; (DYRKP) | 1504 | 5.86 | 160.34 | 551–1498 | Cell-cycle-associated PK DYRK; (T. gondii VEG) | 223 | 2.00 × 10−57 | 63 | ESS33160.1 |
| SRCN_1731 | Cell-cycle-associated kinase GSK | Glycogen synthase kinase (GSK) | 219 | 6.59 | 24.39 | 1–175 | Cell-cycle-associated PK GSK; (H. hammondi) | 330 | 2.00 × 10−112 | 82 | XP_008887193.1 |
| SRCN_1732 | Cell-cycle-associated kinase GSK | Glycogen synthase kinase (GSK) | 203 | 10.78 | 20.72 | 86–203 | CMGC kinase, GSK family TgPK3; (E. brunetti) | 114 | 1.00 × 10−28 | 91 | CDJ46527.1 |
| SRCN_2759 | Cell-cycle-associated kinase partial | Ca2+-dependent PK (CDK); (CRK7) | 1122 | 6.14 | 118.67 | 541–1122 | Cell-cycle-associated PK CDK; (T. gondii VAND) | 118 | 1.00 × 10−25 | 79 | KFH12036.1 |
| SRCN_2845 | CMGC DYRK PRP4 kinase | DYRK; (PRP4) | 1665 | 9.76 | 177.08 | 1267–1596 | Putative PK (CLK3); (P. malariae) | 330 | 1.00 × 10−102 | 69 | SBS85334.1 |
| SRCN_3891 | CMGC kinase | DYRK; (DYRK2) | 674 | 8.85 | 73.85 | 399–674 | Putative CMGC kinase; (T. gondii ME49) | 80.1 | 6.00 × 10−14 | 67 | EPT25192.1 |
| SRCN_4209 | CMGC MAPK family (ERK) MAPK-1 | Mitogen-activated PK (MAPK); (ERK)) | 2361 | 6.73 | 247.71 | 94–754 | CMGC, MAPK/ (ERK) TgMAPK-1; (E. brunetti) | 137 | 1.00 × 10−30 | 74 | CDJ49492.1 |
| SRCN_4674 | Cyclin-dependent kinase | Ca2+-dependent PK (CDK); (CDK7) | 138 | 7.80 | 15.50 | 1–138 | Cyclin-dependent kinase; (T. gondii GT1) | 108 | 7.00 × 10−27 | 58 | EPR60430.1 |
| SRCN_4801 | Cell-cycle-associated kinase | Ca2+-dependent PK (CDK); (CDK5) | 300 | 6.08 | 34.33 | 1–289 | CMGC kinase, CDK family TgPK2; (N. caninum L) | 576 | 0.0 | 91 | XP_003885801.1 |
| SRCN_5365 | Cell-cycle-associated kinase MAPK | Mitogen-activated PK (MAPK; (ERK)) | 417 | 6.77 | 48.32 | 7–363 | Cell-cycle-associated PK MAPK; (H. hammondi) | 823 | 0.0 | 93 | XP_008886907.1 |
| SRCN_6346 | Cell-cycle-associated kinase CDK | Ca2+-dependent PK (CDK); (CDK5) | 690 | 9.55 | 80.90 | 208–603 | Putative cell-cycle-associated PK CDK; (T. gondii ARI) | 390 | 2.00 × 10−122 | 87 | KYF45878.1 |
| SRCN_6427 | CMGC CK2 kinase | Cell Kinase 2 (CK2) | 1395 | 10.29 | 144.86 | 885–1356 | CMGC kinase, CK2 family; (T. gondii MAS) | 241 | 6.00 × 10−73 | 98 | KFH07655.1 |
| SRCN_6472 | Cell-cycle-associated kinase ERK7 | Mitogen-activated PK (MAPK; (ERK)) | 983 | 9.28 | 104.95 | 7–317 | Cell-cycle-associated PK ERK7; (T. gondii ARI) | 647 | 0.0 | 81 | KYF46268.1 |
| SRCN_761 | Cell-cycle-associated kinase | Ca2+-dependent PK (CDK); (CDK7) | 577 | 9.34 | 58.39 | 144–490 | Cell-cycle-associated PK; (H. hammondi) | 283 | 3.00 × 10−88 | 68 | XP_008882409.1 |
| SRCN_895 | Cell-cycle-associated kinase | Ca2+-dependent PK (CDK); (CDK10) | 340 | 8.93 | 38.57 | 1–307 | Cell-cycle-associated PK; (T. gondii ARI) | 234 | 6.00 × 10−75 | 76 | KYF44017.1 |
| SRCN_977 | Cell-cycle-associated kinase CDK | Ca2+-dependent PK (CDK); (PITSLRE/CDK11) | 1502 | 7.38 | 156.35 | 1114–1429 | Cell-cycle-associated PK CDK; (T. gondii p89) | 454 | 3.00 × 10−135 | 92 | KFG28420.1 |
| 5. Kinase Group ‘Other’ (OPK; i.e., kinases with conventional PK (ePK) domains that do not fit into any of the other major groups of kinases) | |||||||||||
| SRCN_108 | Unc-51-like autophagy activating kinase 1 (ULK1) | ULK | 343 | 7.13 | 38.90 | 1-223 | ULK kinase; (T. gondii VAND) | 376 | 2.00 × 10−130 | 75 | KFH07419.1 |
| SRCN_1606 | eIF2 kinase IF2K-C | PEK; (general control nonderepressible 2 (GCN2)) | 4034 | 8.98 | 406.57 | 1235–2178 | eIF2 kinase IF2K-C; (T. gondii VAND) | 259 | 4.00 × 10−67 | 35 | KFH07289.1 |
| SRCN_2076 | Rhoptry kinase family ROP30 | Conserved hypothetical protein | 1276 | 9.18 | 134.73 | 812–1260 | ROP30 (T. gondii VEG) | 230 | 3.00 × 10−63 | 53 | CEL76436.1 |
| SRCN_2123 | Rhoptry kinase family ROP35 | PLK; (PLK-Unclassified) | 291 | 9.30 | 33.50 | 53–265 | ROP35; (T. gondii RUB) | 207 | 2.00 × 10−61 | 43 | KFG59037.1 |
| SRCN_3216 | Rhoptry kinase family ROP32 | CAMK-Unique | 523 | 7.08 | 57.00 | 214–520 | Putative PK; (T. gondii VAND) | 167 | 1.00 × 10−42 | 30 | KFH00232.1 |
| SRCN_2183 | Rhoptry kinase family ROP35 | Aurora-like | 226 | 6.36 | 25.84 | 1–212 | ROP35; (T. gondii VEG) | 198 | 8.00 × 10−59 | 48 | ESS33297.1 |
| SRCN_2271 | Putative PK (incomplete catalytic triad) | NimA (Never in mitosis gene A)-related Kinase (NEK) | 1463 | 9.02 | 157.18 | 437–1145 | Putative PK; (N. caninum L) | 327 | 5.00 × 10−90 | 68 | XP_003881849.1 |
| SRCN_2403 | Aurora kinase (incomplete catalytic triad) | PLK; (SAK/Plk4) | 778 | 9.79 | 79.92 | 492–778 | Putative Aurora kinase; (N. caninum L) | 127 | 3.00 × 10−28 | 44 | XP_003880644.1 |
| SRCN_2630 | NimA related kinase (NEK) family protein | NEK | 351 | 8.70 | 38.38 | 1–336 | NEK kinase; (T. gondii ME49) | 242 | 8.00 × 10−75 | 52 | XP_018638598.1 |
| SRCN_286 | Wee kinase | Inhibitory regulator of the RAS-cAMP (IRA1) kinase suppressor (IKS) | 1019 | 6.20 | 106.67 | 598–959 | Wee kinase; (H. hammondi) | 445 | 5.00 × 10−141 | 58 | XP_008882669.1 |
| SRCN_3075 | Tyrosine kinase-like (TKL) protein | Numb-associated kinase (NAK) | 1571 | 8.41 | 164.18 | 16–500 | TKL; (T. gondii TgCatPRC2) | 138 | 1.00 × 10−32 | 73 | KYK64203.1 |
| SRCN_3142 | PIK3R4 kinase-related | Aurora | 997 | 8.72 | 106.54 | 548–899 | Putative PIK3R4 kinase-related protein; (N. caninum L) | 449 | 2.00 × 10−137 | 60 | XP_003885774.1 |
| SRCN_3151 | NimA related kinase (NEK) family protein | NEK | 3186 | 7.96 | 318.69 | 352–656 | NEK kinase; (T. gondii VEG) | 468 | 7.00 × 10−131 | 73 | CEL78174.1 |
| SRCN_3247 | Rhoptry kinase family ROP27 | Ciliate-D | 345 | 8.94 | 38.81 | 23–325 | ROP27; (T. gondii p89) | 163 | 4.00 × 10−43 | 31 | KFG37427.1 |
| SRCN_3417 | Aurora kinase | Aurora | 438 | 7.65 | 48.49 | 14–289 | Aurora kinase; (T. gondii TgCatPRC2) | 490 | 3.00 × 10−155 | 76 | KYK63669.1 |
| SRCN_3444 | Unc-51-like Autophagy activating kinase 1 (ULK1) | ULK | 406 | 6.52 | 44.69 | 12–406 | ULK kinase; (T. gondii RUB) | 232 | 3.00 × 10−71 | 61 | KFG59767.1 |
| SRCN_3669 | CMGC kinase | ULK | 1803 | 8.41 | 189.65 | 736–1200 | Putative CMGC kinase; (N. caninum L) | 624 | 0.0 | 62 | CEL65030.1 |
| SRCN_4410 | Rhoptry kinase family ROP35 | PKA-like | 204 | 9.44 | 23.50 | 1–166 | ROP35; (H. hammondi) | 107 | 1.00E × 10−25 | 39 | XP_008885989.1 |
| SRCN_4503 | eIF2 kinase IF2K-B | PEK; (general control nonderepressible 2 (GCN2)) | 158 | 5.76 | 17.59 | 1–158 | eIF2 kinase IF2K-B (T. gondii TgCatPRC2) | 149 | 5.00 × 10−41 | 74 | KYK69938.1 |
| SRCN_4528 | NimA related kinase (NEK) family protein | NEK | 187 | 8.20 | 21.23 | 1–186 | NEK kinase; (H. hammondi) | 177 | 4.00 × 10−54 | 64 | XP_008885186.1 |
| SRCN_2404 | Aurora kinase (incomplete catalytic triad) | Serum and glucocorticoid induced Kinase (SGK) | 295 | 8.81 | 31.42 | 1–249 | Putative Aurora kinase; (N. caninum L) | 126 | 7.00 × 10−31 | 43 | CEL65223.1 |
| SRCN_5653 | PEK kinase | Aurora | 626 | 8.27 | 60.75 | 513–626 | PEK kinase (T. gondii TgCatPRC2) | 251 | 1.00 × 10−76 | 60 | KYK62422.1 |
| SRCN_5943 | NIMA-related kinase NIMA1 | NEK | 2842 | 9.04 | 295.44 | 73–383 | NIMA-related PK NIMA1; (T. gondii MAS) | 486 | 2.00 × 10−140 | 67 | KFH05809.1 |
| SRCN_6157 | Unc-51-like autophagy activating kinase 1 (ULK1) | ULK | 2420 | 9.38 | 250.58 | 1380–1672 | ULK kinase (T. gondii ME49) | 99.8 | 3 × 10−21 | 38 | XP_018635814.1 |
| SRCN_6184 | Myosin-light-chain kinase | Ciliate-E2-Unclassified | 478 | 5.42 | 53.65 | 177–474 | ROP19A (T. gondii ME49) | 127 | 3.00 × 10−30 | 27 | XP_018637476.1 |
| SRCN_6572 | Tyrosine kinase-like (TKL) | ULK | 622 | 6.13 | 68.80 | 1–345 | TKL; (T. gondii VAND) | 181 | 1.00 × 10−46 | 74 | KFH00338.1 |
| SRCN_6812 | PK | ULK | 199 | 6.74 | 22.72 | 1–183 | PK; (H. hammondi) | 172 | 3.00 × 10−48 | 53 | XP_008887491.1 |
| SRCN_7083 | Rhoptry kinase family ROP35 | PKA-like | 262 | 9.62 | 30.26 | 1–242 | ROP35; (H. hammondi) | 127 | 6.00 × 10−32 | 39 | XP_008885989.1 |
| SRCN_4310 | Rhoptry kinase family ROP33 | Kinase Homologous to SPS1/STE20 (KHS) | 1591 | 9.85 | 169.63 | 1265–1578 | ROP33; (H. hammondi) | 306 | 2.00 × 10−87 | 39 | XP_008887632.1 |
| SRCN_7082 | Rhoptry kinase family ROP33 | Kinase Homologous to SPS1/STE20 (KHS) | 403 | 9.59 | 45.92 | 77–390 | ROP33 (T. gondii p89) | 277 | 3.00 × 10−89 | 40 | KFG45248.1 |
| SRCN_7084 | Rhoptry kinase family ROP37 | Ribosomal protein S6 Kinases (RSK; (RSK)) | 339 | 5.41 | 38.09 | 19–334 | ROP37; (N. caninum L) | 144 | 1.00 × 10−36 | 36 | CEL64242.1 |
| 6. Kinase Group “Sterile” serine/threonine kinase, or sterile-phenotype kinases (STE) | |||||||||||
| SRCN_1328 | Serine threonine kinase | Conserved hypothetical protein | 1461 | 9.29 | 158.74 | 559–671 | Hypothetical protein, conserved; (E. maxima) | 88.6 | 5.00 × 10−16 | 68 | XP_013335801.1 |
| SRCN_5172 | “Sterile” serine/threonine kinase (STE) | Mammalian Sterile 20-like (MST)) | 6552 | 6.14 | 671.51 | 3410–4122 | STE kinase; (T. gondii TgCatPRC2) | 412 | 1.00 × 10−114 | 54 | KYK71951.1 |
| 7. Kinase Group Tyrosine Kinase-Like (TKL) | |||||||||||
| SRCN_1435 | Tyrosine kinase-like (TKL) | Mixed lineage kinase (MLK); (Leucine Zipper-bearing Kinase (LZK)) | 3064 | 8.05 | 306.74 | 2540–3060 | Tyrosine kinase-like (TKL) protein; (N. caninum L) | 278 | 3.00 × 10−73 | 72 | CEL64955.1 |
| SRCN_1571 | Tyrosine kinase-like (TKL) | Microtubule-associated S/T kinase (MAST) | 550 | 9.76 | 59.91 | 135–501 | Conserved hypothetical protein; (E. praecox) | 76.3 | 6.00 × 10−13 | 53 | CDI87140.1 |
| SRCN_3466 | Tyrosine kinase-like (TKL) | TKL-Unique | 3002 | 9.87 | 320.97 | 2342–2997 | Tyrosine kinase-like (TKL) protein; (H. hammondi) | 202 | 3.00 × 10−50 | 65 | XP_008887506.1 |
| SRCN_3928 | Tyrosine kinase-like (TKL) | LISK - LIMK (LIM kinase) and TESK (Testicular protein Kinase); (DD1) | 5842 | 8.78 | 608.80 | 3639–4268 | Tyrosine kinase-like (TKL) protein; (T. gondii TgCatPRC2) | 216 | 2.00 × 10−61 | 79 | KYK63216.1 |
| SRCN_4277 | Kinase domain-containing protein | TKL-ciliate1 | 2256 | 8.43 | 240.25 | 1570–2256 | Tyrosine kinase-like (TKL) protein; (N. caninum L) | 204 | 8.00 × 10−51 | 61 | CEL67693.1 |
| SRCN_811 | Tyrosine kinase-like (TKL) | TKL-Unique | 1099 | 9.18 | 119.26 | 814–1083 | Putative tyrosine kinase-like (TKL) protein; (E. acervulina) | 403 | 6.00 × 10−125 | 59 | XP_013252162.1 |
| 8. Kinase Group Atypical (aPKs) | |||||||||||
| SRCN_3601 | Atypical MEK-related kinase | Muscle-associated kinase TRIO | 950 | 7.20 | 103.14 | 381–850 | Atypical MEK-related kinase; (T. gondii GT1) | 171 | 1.00 × 10−42 | 32 | EPR62774.1 |
| SRCN_5962 | Atypical MEK-related kinase | Rho-associated protein kinase (ROCK)-like | 805 | 5.01 | 87.71 | 525–805 | Atypical MEK-related kinase; (H. hammondi) | 127 | 4.00 × 10−29 | 56 | XP_008884362.1 |
| SRCN_3988 | Phosphatidylinositol 3-/4-kinase (PI3K) | Atypical/PIKK/ATM | 4251 | 5.95 | 440.5 | 3671–3772 | PI3K, ; (H. hammondi) | 268 | 1 × 10−69 | 55 | XP_008886631.1 |
| SRCN_6465 | Phosphatidylinositol 3-/4-kinase (PI3K) | Atypical PIKK/ATM | 207 | 5.2 | 23.24 | 5-122 | Phosphatidylinositol 4-kinase, partial; (T. gondii p89) | 272 | 4 × 10−93 | 96 | KFG28404.1 |
| SRCN_1259 | Phosphatidylinositol 3-/4-kinase (PI3K) | Atypical/PIKK/FRAP | 1362 | 9.56 | 142.85 | 1140–1311 | PI3K; (T. gondii MAS) | 317 | 3 × 10−94 | 76 | KFH10008.1 |
| SRCN_6464 | Phosphatidylinositol 3-/4-kinase (PI3K) | No hits found | 2108 | 8.95 | 209.68 | 1626–1722 | Phosphatidylinositol 3-4-kinase; (T. gondii p89) | 244 | 1 × 10−63 | 66 | KFG28409.1 |
| SRCN_1743 | Pyruvate dehydrogenase kinase | Atypical/PDHK/BCKDK | 930 | 6.50 | 101.41 | 291–426 | PDHK, isoenzyme-2; (N. caninum L) | 458 | 6 × 10−146 | 45 | CEL70411.1 |
a The protein sequences and their corresponding identified were obtained from the Toxoplasma Genomics Resource database (Release 28; Version May 2016) [42]; b the descriptions of the protein sequence are based on BLASTp annotations using Blast2GO (see the text for details); c the kinase classification is based on BLASTp on the kinase database.