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FRAP recovery for a 10 µm diameter region photobleached at time 0 s. (a) Fl-HDA (white circles) and Fl-DHPE (black circles) on samples that contain 28 nm ROC; (b) Fl-HDA (white circles) and Fl-DHPE (black circles) on samples that contain 55 nm ROC. Error bars are standard deviation; (c) NBD-DHPE fluorescence is quenched by dithionite. Once melittin, a pore forming protein, is added, fluorescence decreases to background levels.
