Figure 5.

Single Biotin-X-DHPE molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. (a) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; (b) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); (c) the distribution of steps observed at curved and flat regions (shown in Figure S3) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.