Figure 4. Depletion of energy and nucleotide metabolite levels.

(A) Decreased levels of key energy-associated metabolites, ATP, NAD⁺ and NADP⁺ induced by colistin, doripenem, and the combination in A. baumannii ATCC 19606. Box plots indicate upper and lower quartiles (top and bottom of box); median (line within box); and the spread of data that are not outliers (whiskers). *≥1.5-log₂-fold, p ≤ 0.05, FDR ≤ 0.1 (one-way ANOVA). (B) Heatmap profile of relative abundance of significantly perturbed nucleotides at (i) 15 min, (ii) 1 hr, and (iii) 4 hr after treatment with colistin (Col), doripenem (Dor) and the combination (Col-Dor) (n = 4). Antibiotics decreased the levels of nucleotides, both purines and pyrimidines, in A. baumannii ATCC 19606.