Figure 5.

Demonstration that chromosome congression is independent of bi-orientation. From A-F, the movement of a polar chromosome along a pre-existing k-fiber is illustrated in a PtK1 cell. The leading kinetochore is indicated (yellow arrows). The kinetochore of a neighbor k-fiber on a bi-oriented chromosome is also indicated (yellow arrowheads). Time is in sec. In G, serial sections of a sliding mono-oriented chromosome with the leading kinetochore laterally attached to a neighbor k-fiber. Kinetochores of the congressing chromosome are indicated (white arrows), as well as the kinetochore of a neighbor k-fiber (black arrowheads). Images adapted from Kapoor et al., 2006 [300]. Reprinted with permission from The American Association for the Advancement of Science (AAAS).