Figure 2.

Different approaches to pangenome assembly. Three genomes (A, B and C) are shown and together they constitute a pangenome. Each genome consists of genomic segments that are marked by the same colour if present in multiple genomes. In the whole de novo assembly approach, the three genomes are assembled individually (a); In the de Bruijn graph approach, the genomes are broken down into segments and the relationships between segments can be traced back to the edges the graph (b); In the iterative mapping and assembly approach, a single genome is used as the basis and reads from other genomes are sequentially mapped and assembled, creating a non-redundant pangenome (c).