Table 2.

Summary of the laboratory tests and assays

Domain	Laboratory parameter		Type of specimen	Method/assay
Immunology	Rotavirus (RV)-specific IgG antibodies		Mother's blood Cord blood Infant's blood	ELISA
	RV-specific IgA antibodies
	Innate immunity activation markers		Infant's blood	TruCulture blood collection and whole-blood culture systems
Virology	Rotavirus shedding		Mother's stool Infant's stool	qPCR
	Poliovirus shedding (type 1 and 3)		Mother's stool Infant's stool	qPCR
Markers of inflammation	Local (gut) inflammation	α-1-Antitrypsin (ATT) Myeloperoxidase	Infant's stool	ELISA
	Systemic inflammation	Acid glycoprotein	Infant's blood	ELISA
Microbiota	Characterisation of microbiota composition		Mother's stool Breast milk Infant's stool	DNA extraction Amplification Next generation Sequencing

qPCR, quantitative PCR.