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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Neuropharmacology. 2016 Nov 27;114:123–134. doi: 10.1016/j.neuropharm.2016.11.021

Figure 5. Corticosterone minimally affects medial CeA responses but blocks ethanol’s effects in females, but not males.

Figure 5

A. Representative evoked glutamatergic-EPSPs (eEPSPs) in CeM at baseline (Control) and during superfusion of 100 nM corticosterone (CORT) and subsequent co-application of 44 mM ethanol (EtOH) onto slices obtained from male (top) and diestrus female (bottom) rats. B. Time course of treatment effects, with CORT treatment following an 8-min baseline and alcohol co-application beginning 20 min after CORT application. Inset shows quantification of the peak CORT and ethanol-induced changes in eEPSPs over a 4-minute bin beginning not less than 15 min into CORT treatment and not less than 6 min into ethanol co-application. Histograms depict mean ± standard error percent change in eEPSP relative to control. C. Quantification of CORT and ethanol effects on I/O responses to 3 intermediate intensity stimuli in males (squares) vs. diestrus females (diamonds). Data are depicted as mean ± standard error percent change in eEPSP after CORT treatment (black and white shapes) and subsequent ethanol co-application (gray shapes), normalized to control. D–E. Histograms depict mean ± standard error paired pulse ratios for male (D) and female (E) cells. n’s = 5–9, as listed on the graph panels; cells becoming unstable after drug wash-on were excluded from I/O analyses, and one female cell with incomplete data was omitted from the time course. *p<0.05 relative to control (one-sample t-test).