Figure 5. 5ZO normalizes SF activation across multiple contexts.

(a) Distribution of β coefficients for three kinase inhibitors across the activated cytokines profiles with values scaled so that −1 represents complete normalization to basal conditions and 0 represents no response to drug. Red dashes represents the median value of the distribution and boxes denote 25^th and 75^th percentile. (b) Effect of 5ZO on SF activated by RA synovial fluid. Blue line shows the level of MCP–1 (left) or GROα (right) in serially diluted synovial fluids alone. The red line depicts cytokine levels in serially diluted synovial fluids following 18 hr incubation with SF and black lines depict the same experiment performed in the presence of 0.6 μM 5ZO. Yellow region highlights the dilution range over which secretion of MCP–1 or GROα by SF is activated by synovial fluid and inhibited by 5ZO. Induction of other 6CK cytokines could not be assayed because they were too abundant in synovial fluid.