Figure 3.

2DE-based screening for acetylated myelin proteins. (A,B) False-colored images of colloidal Coomassie-stained protein in gel (blue, images taken from Figure 2A,F) were superimposed to their corresponding images of AcK immunodetection (orange, images taken from Figure 2D,I). Spots with detectable AcK signal and visibility on colloidal Coomassie-stained gel were labelled with numbers and subjected to protein identification by MS (Table 1). (A’,B’) False- colored images of AcK immune detection (orange, images taken from Figure 2D,I) were overlaid to corresponding images of α-tubulin (blue, images taken from Figure 2E,J). Spot labels were transferred from panels A and B.