Table 2.
Comparison of relative abundance of selected cancer-associated proteins (and their corresponding RNA levels) between UW228 and Daoy. Proteins involved in cell adhesion, actin organization, stress resistance, cell signaling and tumor suppression were found to be upregulated at both transcript and protein levels in UW228 cells relative to Daoy. Conversely, upregulated proteins in Daoy included several proteins involved in signal transduction, cell adhesion and DNA binding/repair. Majority of the amplified transcripts/upregulated proteins in Daoy are localized on chromosome 17, which is frequently gained in several malignancies, including medulloblastoma. (ND—not detected; #—Number).
| Accession | Gene | Description | Chromosome | Daoy | UW228 | ||
|---|---|---|---|---|---|---|---|
| RNA (RPKM) | # Unique Peptides | RNA (RPKM) | # Unique Peptides | ||||
| Overexpressed in Daoy, relative to UW228 | |||||||
| P07900 | HSP90AA1 | Heat Shock Protein 90kDa Alpha Family Class A Member 1 | 14q32 | 421.65 | 18.00 | 167.03 | 7.00 |
| P08238 | HSP90AB1 | Heat Shock Protein 90kDa Alpha Family Class B Member 1 | 6p21 | 775.45 | 19.00 | 362.14 | 9.00 |
| P06748 | NPM1 | Nucleophosmin (Nucleolar Phosphoprotein B23, Numatrin) | 5q35 | 668.25 | 5.33 | 239.64 | 3.00 |
| Q15582 | TGFBI | Transforming Growth Factor Beta Induced | 5q31 | 3135.54 | 17.00 | 1249.52 | 7.00 |
| Q00325 | DSP | Desmoplakin | 6q24 | 22.83 | 10.00 | 0.03 | 2.00 |
| P24821 | TNC | Tenascin C | 9q33 | 241.59 | 8.00 | 37.95 | 1.00 |
| P00533 | EGFR | Epidermal Growth Factor Receptor | 7p11 | 21.24 | 8.00 | 13.13 | 2.00 |
| Q9NS86 | LANCL2 | LanC Lantibiotic Synthetase Component C-Like 2 (Bacterial)1 | 7p11 | 22.28 | 3.00 | 6.52 | 1.00 |
| P62993 | GRB2 | Growth Factor Receptor-Bound Protein 2 | 17q24 | 44.9 | 5.00 | 30.56 | 1.00 |
| P11388 | TOP2A | Topoisomerase (DNA) II Alpha | 17q21 | 129.62 | 8.00 | 45.76 | 0.00 |
| P14923 | JUP | Junction Plakoglobin | 17q21 | 56.97 | 7.00 | 19.84 | 1.00 |
| P46108 | CRK | V-Crk Avian Sarcoma Virus CT10 Oncogene Homolog | 17p13 | 50.20 | 8.00 | 17.16 | 1.00 |
| P04637 | TP53 | Tumor Protein P53 | 17p13 | 235.10 | 5.00 | 36.74 | ND |
| P62826 | RAN | RAN, Member RAS Oncogene Family | 12q24 | 479.63 | 6.00 | 240.29 | 3.00 |
| P43246 | MSH2 | MutS Homolog 2 | 2p21 | 18.07 | 6.00 | 10.87 | ND |
| P52701 | MSH6 | MutS Homolog 6 | 2p16 | 25.35 | 8.00 | 19.98 | 1.00 |
| Overexpressed in UW228, relative to Daoy | |||||||
| P02511 | CRYAB | Crystallin Alpha B | 11q23 | 0.74 | ND | 438.60 | 4.67 |
| Q14847 | LASP1 | LIM And SH3 Protein 1 | 17q12 | 59.55 | 10.00 | 124.73 | 18.00 |
| Q15063 | POSTN | Periostin | 13q13 | 6.53 | ND | 1023.51 | 6.00 |
| P43121 | MCAM | Melanoma Cell Adhesion Molecule | 11q23 | 36.97 | ND | 61.89 | 10.33 |
| P32004 | L1CAM | L1 Cell Adhesion Molecule | Xq28 | 1.15 | ND | 324.27 | 10.33 |
| P46939 | UTRN | Utrophin | 6q24 | 3.7 | 1.00 | 9.86 | 12.00 |
| Q07954 | LRP1 | Low-density lipoprotein receptor-related protein 1 | 12q13 | 6.73 | 2.00 | 79.98 | 13.67 |
| P80723 | BASP1 | Brain Abundant Membrane Attached Signal Protein 1 | 5p15 | 63.7 | 5.00 | 270.37 | 9.00 |
| P98082 | DAB2 | DAB2, Clathrin Adaptor Protein | 5p13 | 7.8 | ND | 68.7 | 2.00 |
| Q6UVK1 | CSPG4 | Chondroitin Sulfate Proteoglycan 4 | 15q24 | 6.8 | 3.00 | 17.8 | 10.00 |
| Q01995 | TAGLN | Transgelin | 11q23 | 27.27 | 8.00 | 353.95 | 12.00 |