Figure 5. miR-126* upregulation decreases shear-resistant firmly adhered Jurkat and THP1 cells to hCMEC/D3 cells.

hCMEC/D3 cells were transfected with control Scrambled Pre-miR (grey) or with Pre-miR-126 (black) (A) Relative miR-126* level expression measured by qRT²-PCR from total RNA. The small nuclear RNA U6 was used as internal control. (B,C) Numbers of shear-resistant firmly adhered THP1 monocytes and Jurkat T cells to (B) unstimulated and (C) cytokine-stimulated hCMEC/D3 cells per field of view (FOV) (640 × 480 μm). Experiments were carried out three times with five FOVs. Data are mean ± SEM. ^#P < 0.05, **P < 0.01, ***^,###P < 0.001, ^#compared to unstimulated.