Table 3.

Relative transcription levels of key glycolytic enzymes in SH5Δpgi_Z and SH5Δpgi_ZG

Gene	SH5Δpgi_Z (0.1 mM)	SH5Δpgi_ZG (0.1 mM)	SH5Δpgi_ZG (0.2 mM)
pgi	0.00	0.00	0.00
zwf	2090.91 ± 146.36	2693.55 ± 212.52	8001.77 ± 400.09
gnd	6.03 ± 0.21	2155.47 ± 73.18	6039.95 ± 259.79
pfkA	2.16 ± 0.11	3.59 ± 0.06	5.48 ± 0.13
pfkB	2.59 ± 0.13	1.29 ± 0.03	1.19 ± 0.03
gapA	34.12 ± 1.54	44.01 ± 0.96	80.95 ± 3.24
edd	1.41 ± 0.02	1.09 ± 0.01	0.87 ± 0.01
tktA	12.20 ± 0.24	5.72 ± 0.12	7.28 ± 0.16
pflB	42.99 ± 1.38	21.51 ± 0.65	37.04 ± 0.74
fhlA	0.38 ± 0.01	0.52 ± 0.01	0.43 ± 0.01
udhA	0.45 ± 0.01	0.55 ± 0.01	0.28 ± 0.01
adhE	4.51 ± 0.05	7.22 ± 0.12	13.76 ± 0.47

The result was from three individual experiment repeats

rpoD was used as the endogenous control and the transcriptional level of rpoD was considered as 1