Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Summary of the purification steps of the cholesterol oxidase produced by Streptomyces aegyptia NEAE 102
| Purification step | Total protein content (mg) | Cholesterol oxidase activity | |||
|---|---|---|---|---|---|
| Total activity (U) | Specific activity (U/mg protein) | Recovery (%) | Purification fold | ||
| Culture filtrate | 3811.27 | 19527.53 | 5.12 | 100 | 1 |
| (NH4) 2SO4, post dialysis | 60.57 | 1217.88 | 20.11 | 6.23 | 3.92 |
| Ion exchange on DEAE Sepharose CL-6B | 31.15 | 501.05 | 16.08 | 2.56 | 3.14 |
