Table 2.
Half life time (T1/2) and heat deactivation constant (k) of cholesterol oxidase produced by Streptomyces aegyptia NEAE 102
Half life time (min) | |
50 °C | 156.25 |
60 °C | 107.27 |
70 °C | 81.82 |
80 °C | 80.33 |
Thermal inactivation rate constant k d 1 (min−1)a | |
50 °C | 0.0044 |
60 °C | 0.0065 |
70 °C | 0.0085 |
80 °C | 0.0086 |
D-value (min) | |
50 °C | 281.25 |
60 °C | 193.09 |
70 °C | 147.28 |
80 °C | 144.60 |
Thermal inactivation rate constant k d 2 (min−1)b | |
50 °C | 0.0025 |
60 °C | 0.0036 |
70 °C | 0.0047 |
80 °C | 0.0048 |
a k d 1 is the deactivation constant after losing 50% of initial activity (at T1/2)
b k d 2 is the deactivation constant after losing 90% of initial activity (at D- value)