Table 4.
Amino acids content of purified cholesterol oxidase produced by Streptomyces aegyptia NEAE 102
| Amino acid | Residue concentration | Estimated no. of residues/mola
(to nearest integer) |
|||
|---|---|---|---|---|---|
| μg/ml | nmol/ml | g/mol | Mol.% | ||
| Hydrophobic | |||||
| Aliphatic | |||||
| Glycine (Gly) | 5.53 | 73.71 | 75.06 | 10.76 | 39 |
| Alanine (Ala) | 7.77 | 87.26 | 89.09 | 12.73 | 46 |
| Isoleucine (Ile) | 3.46 | 26.40 | 131.17 | 3.85 | 14 |
| Leucine (Leu) | 10.28 | 78.71 | 130.67 | 11.49 | 42 |
| Valine (Val) | 3.66 | 31.21 | 117.14 | 4.55 | 16 |
| Aromatic | |||||
| Phenylalanine (Phe) | 5.71 | 34.54 | 165.17 | 5.04 | 18 |
| Charged | |||||
| Acidic | |||||
| Aspartic acid (Asp + Asn) | 5.62 | 42.24 | 133.00 | 6.16 | 22 |
| Glutamic acid (Glu + Gln) | 11.49 | 78.12 | 147.11 | 11.40 | 41 |
| Basic | |||||
| Histidine (His) | 5.91 | 38.10 | 155.17 | 5.56 | 20 |
| Lysine (Lys) | 5.48 | 37.46 | 146.18 | 5.47 | 20 |
| Arginine (Arg) | 8.67 | 49.79 | 174.19 | 7.27 | 26 |
| Polar | |||||
| Serine (Ser) | 4.31 | 40.96 | 105.10 | 5.98 | 22 |
| Threonine (Thr) | 5.27 | 44.26 | 119.11 | 6.46 | 23 |
| Methionine (Met) | 0.33 | 2.21 | 148.42 | 0.32 | 1 |
| Aromatic | |||||
| Tyrosine (Tyr) | 3.67 | 20.28 | 181.16 | 2.96 | 11 |
| Total | 361 | ||||
aThe number of calculated amino acid based on: if the MW of the purified enzyme is 46000 Da and average MW of the 20 most common aa is 110 Da, the no. of aa will be approximately 46000/110 = 418aa. Trp-residues cannot be determined, based on the methodology used. Pro residues were also not detectable. Because of the deletion of 2 of the 20 amino acids in the calculation: W, P (=10%), we have taken away 10% of aa