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. 2017 Mar 29;15:12. doi: 10.1186/s12964-017-0166-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Typical phosphoproteomics workflow. Each step in a phosphoproteomic experiment can contribute to limitations in reproducibility and phosphoproteomic depth, which can ultimately restrict the biological insight obtained from an experiment. Concerted efforts in the phosphoproteomics community to improve each step in this workflow continue to advance our ability to sample the phosphoproteome with greater speed and depth, but comprehensive phosphoproteome coverage remains out of reach. Reproduced from [51] with permission of ACS Publications © 2015