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. 2017 Mar 30;7:45623. doi: 10.1038/srep45623

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Schematic representation of the methodology for identification of proteins in NCCM using mass - spectrometry. (B) Pie-chart showing distribution of ECM proteins identified in NCCM. (C) Peptide signature peaks for CTGF, WISP2 and TGFβ1 observed in mass – spectrometry analysis of NCCM. (D) Immunohistochemistry showing expression of CTGF, WISP-2 and TGFβ1 in paraffin-embedded sections of rat NP (healthy and injured discs) and human degenerative disc NP (Scale bar 50μ).