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. 2017 Jan 23;24(3):519–525. doi: 10.1016/j.sjbs.2017.01.022

Figure 1.

Figure 1

Construction of eukaryotic expression vector pcDNA3.1-EGFP-TERT. (A) The extracted sheep renal tissue RNA under electrophoresis detection; (B) the TERT gene was cloned after PCR amplification; (C) the enzyme-cut plasmid pcDNA3.1-EGFP was re-ligated and transfected with TERT fragments, from which the expressing plasmid was obtained, and the TERT gene (C) was acquired using PCR amplification; (D) The 6132bp band of pcDNA3.1-EGFP and the 1873bp band of TERT (D) were acquired by double enzyme cutting.