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. 2017 Jan 23;24(3):519–525. doi: 10.1016/j.sjbs.2017.01.022

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© 2017 King Saud University

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Adipogenic (A–C) and Neural (D–F) differentiation potential of sheep TERT-BMSCs. (A) Lipid droplets were seen in the cytoplasm of visible after 9 days of culturing. (B) Oil red O-positive cells. (C) Gene expression profile. Lane 1 250/100bp ladder, lane 2 negative control, lane 3 up: PPAR (175bp); down: Leptin (163bp). (D) Condensed cell bodies and extended dendrites were seen after 24-h culture. (E) Toluidine blue staining, (F) gene expression profile. Lane M 250/100bp ladder, lane 2,3 up: NSE (190bp); down: GFAP (123bp), lane 3,4 negative control.