Fig. 3.

Transfection efficiency in relation to particle densities on CHO cells. Summarized in Table 1, Experiment 11. Panel A, transfection efficiency improves as particle density increases in CHO cell-lines. 800 µl-particles are significantly more efficient than 200 µl, F = 0.117, P = 0.007, and 400 µl-particles are significantly more efficient than 200 µl, F = 0.107, P = 0.024. There is no significant difference between 400 µl- and 800 µl-particles (F = 0.579, P = 0.076). Fluorescence images B–D are of transfected CHO cells with 200 µl (B), 400 µl (C) and 800 µl (D) of particles in 2 ml medium. * indicates p-value < 0.05 and ** indicates p-value < 0.01. Bars in B–D are 150 µm.