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. 2017 Feb 11;24(3):622–629. doi: 10.1016/j.sjbs.2017.01.034

Fig. 4.

Fig. 4

Influence of glycerol treatment on CHO and C2C12 cells transfection efficiency. Summarized in Table 1, Experiment 9. Panel A, glycerol shock generated significantly more efficient transfection when used with HEPES buffer saline and HBS buffer saline in both CHO and C2C12 cell lines (F = 0.003, P = 0.044 with HEPES in CHO, F = 0.138, P = 0.002 with HBS in CHO, F = 0.082, P = 0.00003 with HEPES in C2C12, and F = 0.092, P = 0.008 with HBS in C2C12). Panels B–E show CHO cells, comparison of transfection efficiency between glycerol shock (B) and no shock (C) in HEPES buffer saline and comparison of transfection efficiency between glycerol shock (D) and no shock (E) in HBS buffer saline. Panels F-I show C2C12 cells, comparison of transfection efficiency between glycerol shock (F) and no shock (G) in HEPES buffer saline and comparison of transfection efficiency between glycerol shock (H) and no shock (I) in HBS buffer saline. * indicates p-value < 0.05, ** indicates p-value < 0.01, and *** indicates p value < 0.001. Bars in B–I are 150 µm.