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Btub-Bklc interaction (A). Coomassie stained SDS-PAGE showing that Bklc reversibly binds to Btub polymers in co-sedimentation assays (B). Schematic representation of the experimental procedure corresponding to fractions S1, P and S2 of the co-sedimentation assay (C). Ni-NTA-gold labeling of Bklc bound to Btubs in cryo-electron microscopy (left) Btub filaments without Bklc (right) Scale bar: 100 nm.
