Table 5.
Antibodies and chemicals used for the immunohistochemistry.
| Histone H3 Acetyl K9 1 | Histone H3 Tri Methyl K4 2 |
|---|---|
| Blocking solution 3: 5 min | Blocking solution 3: 5 min |
| primary antibody 1: 1:200 in PBS 5, incubation: 16 h, 4 °C | primary antibody 2: 1:500 in PBS 5, incubation: 16 h, 4 °C |
| PostBlock 3: 20 min | PostBlock 3: 20 min |
| HRP Polymer 3: 30 min | HRP Polymer 3: 30 min |
| Chromogen: DAB 4 (0.5 min) | Chromogen: DAB 4 (1 min) |
1 Anti Histone H3 acetyl K9, clone Y28 (rabbit IgG), concentration: 0.059 mg/mL, company: Abcam (Cambridge, UK), order number: ab32129; 2 Anti Histone H3 tri methyl K4, rabbit IgG polyclonal, concentration: 1 mg/mL, company: Abcam, order number: ab8580; 3 ZytoChem Plus HRP Polymer Kit (Mouse/Rabbit) 3 × 100; company: Zytomed Systems (Berlin, Germany) Nr. POLHRP-100; 4 Liquid DAB + Substrate Chromogen System 1 mg/mL, DAKO; 5 Dulbecco’s Phosphate Buffered Saline.