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. 2017 Feb 24;18(3):487. doi: 10.3390/ijms18030487

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A2 — AsPC-1 expresses tissue factor, and causes platelet aggregation (A) Light transmission aggregometry showed that AsPC-1-induced aggregation of human washed (HW) platelets, which was tissue factor-dependent, as addition of low-molecular-weight heparin (LMWH) prevented platelet aggregation. The downward-slope represents light transmission approaching 100%, which is indicative of platelet aggregation; (B) Flow cytometry analysis of AsPC-1 cells stained with anti-tissue factor show AsPC-1 expressing tissue factor above isotype control; (C) Phase-contrast images show platelets aggregating (black arrow) around AsPC-1 cells.