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. 2017 Feb 25;18(3):497. doi: 10.3390/ijms18030497

Figure 5.

Figure 5

Identification of Tri5 gene: (A) expression and purification of trichodiene synthase encoded by Tri5 gene from M. roridum: M. Protein Marker; 1. Un-induced sample; 2. Total proteins of induced samples; 3. Supernatant of induced sample; 4. 55 mM imidazole eluate; and 5. 100 mM imidazole eluate; and (B) Western blot analysis of trichodiene synthase encoded by Tri5 gene from M. roridum: 1. Total proteins of un-induced sample; 2. Supernatant of induced sample; and 3. 55 mM imidazole eluate.