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NMR characteristics of the CXCL7 monomer. (A) The HSQC spectrum shows excellent chemical shift dispersion indicating a well-folded single species at pH 4.0. The folded arginine side chain peaks are indicated in closed brackets; (B,C) Large chemical shift changes observed as a function of pH are shown for M6 and K17. The transition is from pH 4.2 (black), 4.4 (purple), 5.0 (blue), 5.5 (green) 6.0 (orange) to 7.0 (red).
