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. 2017 Feb 27;18(3):513. doi: 10.3390/ijms18030513

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of myeloma cell-derived microvesicles (MM-MVs) (A,B) Transmission electron microscopy revealing MVs (arrow) as 100–1000 μm vesicles shed from U266 cells (U266-MVs). Scale bar = 2 μm (A) and 0.2 μm (B); (C) Scanning electron microscopy showing typical morphology of MVs derived from RPMI8226 myeloma cells (RPMI8226-MVs). Scale bar = 2 μm; (D,E) Representative flow cytometry analysis of U266-MVs and RPMI8226-MVs revealing the presence of CD138+Calcein-AM+ vesicles, with the 1 μm microbeads for gating the MVs for size verification and Calcein-AM to detect the integrity of MVs.

Characterization of myeloma cell-derived microvesicles (MM-MVs) (A,B) Transmission electron microscopy revealing MVs (arrow) as 100–1000 μm vesicles shed from U266 cells (U266-MVs). Scale bar = 2 μm (A) and 0.2 μm (B); (C) Scanning electron microscopy showing typical morphology of MVs derived from RPMI8226 myeloma cells (RPMI8226-MVs). Scale bar = 2 μm; (D,E) Representative flow cytometry analysis of U266-MVs and RPMI8226-MVs revealing the presence of CD138+Calcein-AM+ vesicles, with the 1 μm microbeads for gating the MVs for size verification and Calcein-AM to detect the integrity of MVs.