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Demethylation restored the function of -1C κB sites. U937 and HEK 293T cells that do not express TFPI2 or IRF7, respectively, were treated with or without 5-Aza-CdR for three days. TFPI2 or IRF7 expression upon TNFα stimulation was then analyzed by reverse transcription PCR (RT-PCR). GAPDH expression was used as internal controls. Data shown are the results of one of three experiments.
