Table 3.
Average metal (loid) concentrations ± standard deviation (SD) after addition of the reconstituted mixtures to the wells. BDL = below detection limit. Standard embryo medium was used as a negative and internal control. Sampling points are in the same sequence as the contamination gradient.
| µg/L (% of Nominal) | Al | Mn | Fe | Ni | Cu | Zn | As | Cd |
|---|---|---|---|---|---|---|---|---|
| SN1 (n = 3) | 52 ± 0 (98.4%) | 30.9 ± 0.4 (99.1%) | 253 ± 5 (95.1%) | 2.6 ± 0.09 (109.9%) | 12.3 ± 0.2 (97.8%) | 64.3 ± 1.1 (97.7%) | 2.4 ± 0.09 (92.8%) | 0.6 ± 0.04 (107.2%) |
| D (n = 2) | 926 ± 10 (102.4%) | 5446 ± 50.2 (102.8%) | 10582 ± 842 (101.4%) | 64.6 ± 0.02 (96.1%) | 2.9 ± 0.3 (98.3%) | 98550 ± 1089 (99.9%) | 128 ± 0.6 (97.9%) | 3290 ± 58.7 (97.2%) |
| 1/10D (n = 3) | 93 ± 1 (102.3%) | 523 ± 3.2 (98.7%) | 1069 ± 49 (102.4%) | 6.9 ± 0.09 (102.9%) | BDL | 9691 ± 84.1 (98.3%) | 12.8 ± 0.08 (98.0%) | 342 ± 7.8 (101.2%) |
| 1/50D (n = 3) | 18 ± 0 (99.6%) | 98.8 ± 2.3 (93.3%) | 196 ± 14 (93.8%) | 1.5 ± 0.2 (109.9%) | BDL | 1854 ± 52.1 (94.0%) | 2.6 ± 0.07 (101.2%) | 63.9 ± 2.5 (94.4%) |
| SN2 (n = 3) | 82 ± 1 (97.6%) | 257 ± 1.7 (97.5%) | 672 ± 7 (94.7%) | 4.8 ± 0.1 (97.1%) | 4.8 ± 0.2 (112.6%) | 3256 ± 27.1 (94.4%) | 2.7 ± 0.04 (94.1%) | 6.9 ± 0.08 (102.7%) |
| Standard embryo medium | BDL | BDL | 10 | 0.1 | 1.3 | 5.1 | BDL | BDL |
| Control SN1 | BDL | BDL | BDL | 0.9 | BDL | BDL | BDL | BDL |
| Control D | BDL | BDL | BDL | 0.5 | BDL | BDL | BDL | BDL |
| Control SN2 | BDL | BDL | BDL | 0.6 | BDL | BDL | BDL | BDL |