Table 2.
Real-time quantitative polymerase chain reaction results.
| miRNA Species | HARS without Pancytopenia | HARS with Pancytopenia | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. Samples Evaluated | Mean | SD | Min. | Max. | No. Samples Evaluated | Mean | SD | Min. | Max. | FC | Student’s t-test | |
| screening | ||||||||||||
| miR-425 | 5 | 7.2 | 0.4 | 6.5 | 7.5 | 4 | 8.1 | 0.3 | 7.7 | 8.4 | 0.5 | 0.005 |
| miR-192 | 5 | 12.7 | 0.4 | 12.4 | 13.2 | 4 | 14.2 | 0.9 | 13.1 | 15.1 | 0.4 | 0.01 |
| miR-149-raute | 5 | 14.4 | 0.5 | 13.8 | 15.0 | 4 | 13.4 | 0.5 | 12.9 | 14.0 | 2.0 | 0.02 |
| miR-598 | 3 | 19.5 | 0.1 | 19.4 | 19.7 | 2 | 19.0 | 0.0 | 19.0 | 19.1 | 1.4 | 0.02 |
| miR-30c | 5 | 5.3 | 0.7 | 4.2 | 5.8 | 4 | 6.7 | 0.9 | 5.6 | 7.6 | 0.4 | 0.03 |
| miR-1290 | 5 | 17.2 | 0.7 | 16.5 | 18.1 | 4 | 16.1 | 0.4 | 15.7 | 16.5 | 2.1 | 0.03 |
| miR-15b-raute | 5 | 8.8 | 0.8 | 8.1 | 10.2 | 4 | 10.3 | 1.0 | 9.6 | 11.7 | 0.4 | 0.04 |
| miR-222 | 5 | 6.9 | 0.5 | 6.3 | 7.6 | 4 | 7.7 | 0.4 | 7.3 | 8.1 | 0.6 | 0.04 |
| miR-942 | 5 | 8.7 | 0.6 | 8.0 | 9.5 | 4 | 9.9 | 0.9 | 9.0 | 11.2 | 0.4 | 0.05 |
| validation | ||||||||||||
| miR-425 | 7 | 7.1 | 0.4 | 6.6 | 7.9 | 4 | 8.1 | 0.3 | 7.7 | 8.4 | 0.5 | 0.002 * |
| miR-192 | 7 | 13.1 | 0.9 | 12.2 | 14.9 | 4 | 14.2 | 0.9 | 13.1 | 15.1 | 0.5 | 0.09 |
| miR-149-raute | 7 | 13.4 | 0.5 | 12.7 | 14.2 | 4 | 13.4 | 0.5 | 12.9 | 14.0 | 1.0 | 0.9 |
| miR-598 | 5 | 19.2 | 0.9 | 18.1 | 20.2 | 2 | 19.0 | 0.0 | 19.0 | 19.1 | 1.1 | 0.7 |
| miR-30c | 7 | 5.8 | 0.9 | 4.6 | 7.0 | 4 | 6.7 | 0.9 | 5.6 | 7.6 | 0.5 | 0.1 |
| miR-1290 | 7 | 16.6 | 0.4 | 16.0 | 17.1 | 4 | 16.1 | 0.4 | 15.7 | 16.5 | 1.3 | 0.2 |
| miR-15b-raute | 7 | 9.2 | 1.1 | 8.0 | 11.4 | 4 | 10.3 | 1.0 | 9.6 | 11.7 | 0.5 | 0.1 |
| miR-222 | 7 | 7.3 | 1.1 | 5.7 | 9.2 | 4 | 7.7 | 0.4 | 7.3 | 8.1 | 0.7 | 0.4 |
| miR-942 | 7 | 9.3 | 0.7 | 8.3 | 10.2 | 4 | 9.9 | 0.9 | 9.0 | 11.2 | 0.6 | 0.2 |
| validation on all samples | ||||||||||||
| miR-425 | 12 | 7.1 | 0.4 | 6.5 | 7.9 | 4 | 8.1 | 0.3 | 7.7 | 8.4 | 0.5 | 0.0003 * |
| miR-192 | 12 | 13.0 | 0.7 | 12.2 | 14.9 | 4 | 14.2 | 0.9 | 13.1 | 15.1 | 0.4 | 0.02 |
| miR-149-raute | 12 | 13.8 | 0.7 | 12.7 | 15.0 | 4 | 13.4 | 0.5 | 12.9 | 14.0 | 1.3 | 0.3 |
| miR-598 | 8 | 19.3 | 0.7 | 18.1 | 20.2 | 2 | 19.0 | 0.0 | 19.0 | 19.1 | 1.2 | 0.6 |
| miR-30c | 12 | 5.6 | 0.8 | 4.2 | 7.0 | 4 | 6.7 | 0.9 | 5.6 | 7.6 | 0.5 | 0.03 |
| miR-1290 | 12 | 16.8 | 0.7 | 16.0 | 18.1 | 4 | 16.1 | 0.4 | 15.7 | 16.5 | 1.6 | 0.07 |
| miR-15b-raute | 12 | 9.0 | 1.0 | 8.0 | 11.4 | 4 | 10.3 | 1.0 | 9.6 | 11.7 | 0.4 | 0.04 |
| miR-222 | 12 | 7.1 | 0.9 | 5.7 | 9.2 | 4 | 7.7 | 0.4 | 7.3 | 8.1 | 0.7 | 0.2 |
| miR-942 | 12 | 9.1 | 0.7 | 8.0 | 10.2 | 4 | 9.9 | 0.9 | 9.0 | 11.2 | 0.5 | 0.06 |
The table summarizes real-time quantitative polymerase chain reaction (qRT-PCR) results (normalized threshold cycle (CT)-values) of microRNAs (miRNAs) examined during the screening and validation (remaining samples and with all samples combined) stage. Descriptive statistics reflect the distribution and the fold change (FC) difference among miRNAs differentially expressed relative to mean HARS 0 severity degree (H0) as the reference and between both haematologic acute radiation syndrome (HARS) groups in order to compare discrimination between HARS without and with pancytopenia. Numbers for columns entitled “No. Samples Evaluated” reflect the total number of blood sample examined per group. Depending on the miRNA this number can be smaller. The Student’s t-test was performed for comparison of mean FC between the two HARS groups. p-values corrected for multiple comparisons (Bonferroni) are marked with an asterisk when they reached values of p < 0.006 (p = 0.05/9 genes/hypothesis). SD: Standard deviation.