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. 2017 Mar 3;18(3):547. doi: 10.3390/ijms18030547

Figure 5.

Figure 5

Analysis of the interaction between uL3 and xCT and GST-α1 gene promoters. (A) Protein samples of DNA-uL3 or DNA-IgG immunocomplexes from Calu-6 cells untreated or treated with 10 μM 5-FU for 48 h were analysed by Western blotting with antibodies against uL3. Note the absence of signal in the DNA-IgG immunocomplex. The same DNA-immunoprecipitates were subjected to qPCR with primers specific for xCT or GST-α1 gene promoters. The quantification of signals is shown. *p < 0.05 vs. DNA levels in untreated cells set at 1. Calu-6 cells treated or not treated with 10 μM 5-FU for 48 h, uL3ΔCalu-6 and rCalu-6 cells were transiently transfected with (B) xCT promoter luciferase reporter plasmid or (C) GST-α1 promoter luciferase reporter plasmid in presence or absence of Nrf2 siRNA or pHA-uL3 plasmid. Analysis of the relative luciferase activity, normalized against Renilla Luciferase (pRL) activity, of the samples is shown. ** p < 0.01, * p < 0.05 vs. untreated Calu-6 cells set at 1.