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. 2017 Mar 7;18(3):564. doi: 10.3390/ijms18030564

Figure 1.

Figure 1

Cyclic mechanical stretch could upregulate the expression of Rspo1 in BMSCs and MC3T3-E1 cells. (A) qRT-PCR analysis of Rspo1 in BMSCs during osteogenic differentiation; (B) qRT-PCR analysis of Rspo1 in BMSCs treated with cyclic mechanical stretch (CMS) at different elongation (representative illustration for three days after CMS) and (C) for different time periods (representative illustration for CMS at 5% elongation); (D) Western blot analysis and (E) ELISA analysis of Rspo1 in BMSCs treated with CMS (at 5% elongation for three days); (F) Western blot analysis, (G) qRT-PCR analysis and (H) ELISA analysis of Rspo1 in MC3T3-E1 cells treated with CMS (at 5% elongation for three days); (I) qRT-PCR analysis of osteogenic differentiation marker genes (Ocn, ALP, Col-1a1) and Rspo1 in BMSCs under CMS (5% elongation) or (J) treated with BMP2 for three days; (K) ELISA measurement for supernatant Rspo1 in BMSCs treated with BMP2 for three days; (L) qRT-PCR analysis and (M) supernatant level of Rspo2 in BMSCs treated with CMS (at 5% elongation for three days). Data are presented as the mean ± SD, * p < 0.05, ** p < 0.01. p-Values in (A–C,I,J) are based on ANOVA and post hoc analysis, and the other p-values were based on Student’s t-test. All results are representative of at least three independent experiments. Diff: osteogenic differentiated; Undiff: undifferentiated; Con: static control; CMS: cyclic mechanic stretch; NS: not significant; BMP2: bone morphogenetic protein 2; ELISA: enzyme linked immunosorbent assay.