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GLOI knockdown suppresses expression of GLOI mRNA, protein, and enzyme activity in colorectal cancer (CRC) cells. The expression of GLOI mRNA (A), GLOI protein (B), and GLOI enzyme activity (C) was lower in SW480, SW620, DLD-1, and HCT-15 CRC cells transfected with short hairpin GLOI (shGLOI) than in cells transfected with short hairpin empty vector (shNC). * p < 0.05, ** p < 0.01, *** p < 0.001. All data are representative of three independent experiments (n = 3).
