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. 2017 Mar 7;18(3):581. doi: 10.3390/ijms18030581

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Methyl gallate restores the lipopolysaccharide (LPS)-mediated bone erosion mice model. (A) Mice were euthanized on day 8 after first LPS injection and 2D or 3D radiographs of the coronal and transverse sections of the proximal femurs were obtained with a micro-CT scanner; (B) The BV/TV, Tb.Sp, Tb.Th, and Tb.N of the femurs were determined by analyzing the micro-CT data in the INFINITT-Xelis software. * p < 0.05; ** p < 0.01; ^## p < 0.01; ^### p < 0.001; (C) Dissected femurs were fixed, decalcified, embedded, and sectioned. The slices were stained with TRAP (bottom) and hematoxylin & eosin (H&E) (top), and photographed under a light microscope at the indicated magnification (10×); (D) The number of osteoclasts per visual field of tissues was measured by histomorphometric analysis. *** p < 0.01; ^## p < 0.01.