Table 1.
Oligonucleotide sequences for primers used in this study.
| Primer Name | Primer Sequence (5′–3′) | Purpose |
|---|---|---|
| pLOX3-SP1 | AACTCAGTGTGGTGAAGATTGCGGATG | |
| pLOX3-SP2 | AAGGCGACATCGAAAGCTGAATCTCC | DkLOX3 promoter clone |
| pLOX3-SP3 | CAAGTAAGCTGCCTTCCCAAGCTTCC | |
| DkLOX3-PF | CCCAAGCTTCCACAATTATTCTTTGGTTATTTCG | DkLOX3 full-length promoter clone |
| DkLOX3-PR | GCTCTAGACTTCTTCTTCTTCTTCTTCTTCTGC | |
| DkLOX3-PF5 | CCCAAGCTTTATAGTATGGTCTATCATTCTCATACG | DkLOX3 promoter deletion derivatives construct |
| DkLOX3-PF4 | CCCAAGCTTGTACCCCATGTTTGATACAACTCT | |
| DkLOX3-PF3 | CCCAAGCTTGCATTTCTATGTATGCTCTCTTATG | |
| DkLOX3-PF2 | CCCAAGCTTCTGGATCTAGTTCAACCCATAAG | |
| DkLOX3-PF1 | CCCAAGCTTTTATCAGGGATCTGATTTGTCTTA | |
| DkLOX3qF | CACTGCTCTTCCCTACCA | DkLOX3 qRT-PCR |
| DkLOX3qR | CAGAGGGAGAAATCAGTGATACAC | |
| ActinqF | GGATTCTGGTGATGGTGTTAG | Actin qRT-PCR |
| ActinqR | CAGCAGTTGTTGTGAAGGAGT |
Letters “F” and “R” indicate the forward and reverse primers, respectively. Underlined sequences show restriction enzyme sites.