Table 3.
Triacylglicerol (TAG) molecular species content in the isolated lipid fraction F2 obtained from buttermilk ordered by carbon number (CN) and double bonds (DB) as determined by UPLC/QToF-MS.
| Time (min) | Exact Mass | Content (%) | CN/DB | Molecular Species |
|---|---|---|---|---|
| 0.93 | 470.3616 | 0.18 | 24:0 | TAG(8:0/8:0/8:0) |
| 0.97 | 578.4507 | 0.15 | 32:2 | TAG(14:1/14:1/4:0) |
| 0.98 | 496.3795 | 0.08 | 26:1 | TAG(14:1/8:0/4:0) |
| 1.03 | 498.3913 | 0.64 | 26:0 | TAG(14:0/8:0/4:0) |
| 1.07 | 524.4069 | 0.29 | 28:1 | TAG(16:1/8:0/4:0) |
| 1.13 | 526.4207 | 1.02 | 28:0 | TAG(16:0/8:0/4:0) |
| 1.45 | 608.4994 | 1.49 | 34:1 | TAG(16:1/14:0/4:0) |
| 1.68 | 636.5303 | 2.41 | 36:1 | TAG(14:0/14:1/8:0) |
| 1.72 | 610.5145 | 5.58 | 34:0 | TAG(16:0/12:0/6:0) |
| 1.73 | 662.5461 | 0.84 | 38:2 | TAG(16:1/16:1/6:0) |
| 1.96 | 690.5734 | 1.06 | 40:2 | TAG(14:1/14:1/12:0) |
| 2.03 | 638.5447 | 7.60 | 36:0 | TAG(12:0/12:0/12:0) |
| 2.40 | 666.5769 | 8.81 | 38:0 | TAG(16:0/16:0/6:0) |
| 2.67 | 772.6514 | 0.21 | 48:3 | TAG(16:1/16:1/16:1) |
| 2.67 | 720.6229 | 3.36 | 42:1 | TAG(18:1/16:0/8:0) |
| 2.70 | 694.6105 | 7.59 | 40:0 | TAG(16:0/16:0/8:0) |
| 2.95 | 734.6395 | 0.46 | 43:1 | TAG(14:0/14:1/15:0) |
| 3.15 | 774.6678 | 1.00 | 46:2 | TAG(16:1/16:0/14:1) |
| 3.18 | 748.6538 | 4.51 | 44:1 | TAG(16:0/14:0/14:1) |
| 3.25 | 722.6372 | 0.71 | 42:0 | TAG(16:0/14:0/12:0) |
| 3.53 | 788.6851 | 0.01 | 47:2 | TAG(14:1/15:0/18:1) |
| 3.84 | 802.6989 | 1.50 | 48:2 | TAG(16:0/16:1/16:1) |
| 4.71 | 830.7292 | 2.97 | 50:2 | TAG(16:1/18:1/16:0) |
| 3.87 | 776.6866 | 4.39 | 46:1 | TAG(18:1/14:0/14:0) |
| 4.30 | 790.7012 | 1.67 | 47:1 | TAG(18:1/14:0/15:0) |
| 4.37 | 764.6894 | 1.55 | 45:0 | TAG(16:0/15:0/14:0) |
| 4.71 | 856.7448 | 1.37 | 52:3 | TAG(16:0/18:1/18:2) |
| 4.77 | 804.7145 | 6.17 | 48:1 | TAG(14:0/18:1/16:0) |
| 4.87 | 778.6984 | 3.13 | 46:0 | TAG(14:0/14:0/18:0) |
| 5.12 | 818.7254 | 1.79 | 49:1 | TAG(15:0/16:0/18:1) |
| 5.19 | 792.7154 | 0.75 | 47:0 | TAG(16:0/14:0/17:0) |
| 5.33 | 818.7254 | 0.69 | 49:1 | TAG(16:0/18:1/15:0) |
| 5.43 | 792.7154 | 0.80 | 47:0 | TAG(16:0/16:0/15:0) |
| 5.94 | 832.7463 | 7.04 | 50:1 | TAG(16:0/16:0/18:1) |
| 6.05 | 806.7285 | 3.19 | 48:0 | TAG(16:0/16:0/16:0) |
| 6.19 | 828.7155 | 0.28 | 50:3 | TAG(18:2/18:1/14:0) |
| 6.22 | 884.7746 | 1.13 | 54:3 | TAG(18:1/18:1/18:1) |
| 6.37 | 872.7741 | 0.72 | 53:2 | TAG(18:1/18:1/17:0) |
| 6.40 | 846.7602 | 1.41 | 51:1 | TAG(16:0/17:0/18:1) |
| 6.46 | 820.7466 | 1.13 | 49:0 | TAG(16:0/16:0/17:0) |
| 6.81 | 820.7466 | 0.89 | 49:0 | TAG(16:0/15:0/18:0) |
| 7.39 | 834.7604 | 0.19 | 50:0 | TAG(16:0/16:0/18:0) |
| 7.50 | 886.7917 | 2.60 | 54:2 | TAG(18:0/18:1/18:1) |
| 7.64 | 860.7755 | 5.50 | 52:1 | TAG(16:0/18:0/18:1) |
Species detected as [M + NH4]+ and [M + NH4 + H3CCN]+.