Comparison of viability in cell populations between STH and MH in HCT-116 cells by Tali™ Image-Based Cytometer (A–C). After 24 h of cell seeding, HCT-116 cells were treated with STH (0, 3, 6, 9 and 12 mg/mL) and MH (0, 5, 10, 15 and 20 mg/mL) for 24, 48 and 72 h at which time approximately 80% to 40% cells were alive. Cell viability was measured by using Tali™ Viability Kit assay and results were expressed as a percentage (%) of live and dead cells. Data are shown as the mean ± SD of three experiments. Different superscripts letter for each column indicated significant differences (p < 0.05).