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. 2017 Mar 11;18(3):613. doi: 10.3390/ijms18030613

Figure 6.

Figure 6

STH and MH induce ROS generation in HCT-116 cells. HCT-116 cells were treated with or without different concentrations of STH (0, 3, 6, 9 and 12 mg/mL) and MH (0, 5, 10, 15 and 20 mg/mL)for 24, 48 and 72 h. Intracellular ROS levels were calculated by using CellROX® Orange assay kit and the Tali™ Image-based Cytometer (A). Image-Based cytometry was used to quantify ROS induction (% of propidium iodide (PI) positive) in HCT-116 cells following STH and MH treatment at 48 h (B). The blue line of the thumbnail histogram indicated the set threshold. Representative fluorescence image of HCT-116 cells shows the effect of STH and MH treatment at 48 h: non-fluorescent while in a reduced state and bright red fluorescence upon oxidation by ROS. Scale bar = 50 µm, arrows indicate single cell (cell size = 10 µm). Data are shown as the mean ± SD of three experiments. Columns associated with the same set of data with different symbolic letters are significantly different (p < 0.05) from controls.