Figure 3.

Effect of β-glucan on CXCL-8 release. (a) Curdlan (10 µg/mL) or laminarin (10 µg/mL) was added to BEAS-2B cells for 1 h prior to stimulation with 100 µg/mL of F. proliferatum extracts for 24 h. The supernatant was collected and the secretion of CXCL-8 determined by ELISA; (b) BEAS-2B cells were pre-incubated with curdlan at the indicated concentrations for 1 h. Cells were then exposed to F. proliferatum extracts for 24 h and the amounts of CXCL-8 in the cultured supernatants were determined by ELISA. The results are representative of three (a) and two (b) independent experiments performed in duplicate. Asterisks (*) indicate significant differences (p < 0.05) between the paired samples.