Figure 4.

Effect of the anti-Dectin 1 and Syk specific inhibitor piceatannol on F. proliferatum stimulated CXCL-8 release. BEAS-2B cells were pre-incubated in the absence or presence of mouse monoclonal antibody against human Dectin-1 (2 µg/mL) (a) or Syk inhibitor piceatannol (5 μM) (b) for 1 h. Cells were then exposed to F. proliferatum extracts for 24 h and cultured supernatants were collected for CXCL-8 determination by ELISA. Results are representative of three independent experiments performed in duplicate.