Figure 3.

Effect of SHM on UVB-induced MMP-1 transcription in cultured primary human dermal fibroblasts: (A) Expression of MMP-1 was determined by Western blot. MMP-2 was used as a loading control. Cells were pretreated with SHM at the indicated concentrations for 1 h, and then further treated with 0.02 J/cm² UVB for 48 h at 37 °C. MMP-1 expression data were quantified using Image J software analysis. Data (n = 3) represent the means ± SD; (B) MMP-1 mRNA levels for the SHM group were analyzed by real-time quantitative PCR. Cells were pretreated with SHM at the indicated concentrations for 1 h, and then further treated with 0.02 J/cm² UVB for 48 h at 37 °C. Data (n = 3) represent the means ± SD; (C) MMP-1 transactivation by SHM, measured using a luciferase reporter gene assay as described in the Materials and Methods. Cells were pretreated with SHM at the indicated concentrations for 1 h, and then further treated with 0.02 J/cm² UVB for 24 h at 37 °C. Data (n = 3) represent the means ± SD; (D) Cell viability after SHM treatment. Viability was measured using an MTT assay as described in the Materials and Methods. Cells were pretreated with SHM at the indicated concentrations for 48 h at 37 °C. Data (n = 4) represent the means ± SD. Means with letters a–c within a graph are significantly different from each other at p < 0.05. Means with letters ## within a graph are significantly different between untreated control and UVB treated group at p < 0.05.