Figure 4.

Effect of SHM on UVB-induced AP-1 protein expression and signaling pathways in cultured primary HDF and TIMP-1 cell lines. (A) AP-1 transactivation by SHM was measured using a luciferase reporter gene assay as described in the Materials and Methods. Cells were pretreated with SHM at the indicated concentrations for 1 h, and then further treated with 0.02 J/cm² UVB for 36 h at 37 °C. Data (n = 3) represent the means ± SD; Means with letters ## within a graph are significantly different between untreated control and UVB treated group at p < 0.05; (B–D) Phosphorylated and total protein levels were conducted by Western blot using specific antibodies. Cells were pretreated with SHM at the indicated concentrations for 1 h, and then further treated with 0.02 J/cm² UVB for 30 min at 37 °C.