Figure 3.
Intracellular internalization and cytotoxicity of AQ4N-hCe6-liposome. (a) CLSM observation of intracellular internalization profiles of AQ4N-hCe6-liposome, plain hCe6-liposome, and free AQ4N after incubation with 4T1 cells. DAPI, Ce6, and AQ4N were excited at 404, 488, and 633 nm, respectively. (b) Cytotoxicity of AQ4N-hCe6-liposome to 4T1 cells under normoxia and hypoxia conditions measured by the standard MTT assay. The error bars were based on triplicate measurements. (c) Cytotoxicity of AQ4N-hCe6-liposome to 4T1 cells in the presence or absence of 660 nm LED light irradiation recorded by the standard MTT assay. The error bars were based on triplicate measurements.