Figure 4. Fecal Transplant Aids in the Recovery of the Host in a Microbiota-Independent Fashion.

(A) Clindamycin-treated mice were gavaged with C. difficile or PBS control. Fecal transplants from conventional mice were given to both infected and uninfected mice and all were tracked for 19 more days.

(B) Alpha diversity of mice in each treatment group, starting at 2 days prior to fecal transplant as measured by the PD_whole_tree metric (mean ± SEM).

(C) Principal-component analysis of host proteins, plotted by time point.

(D) Expression profile of REG3γ throughout the course of the entire experiment (mean ± SEM).

(E) Cluster analysis of the proteins that significantly differentiate the antibiotic-treated mice at day 24 from the rest. Symbols under the column refer to the treatment group as indicated in (C).

(F) Model of host recovery as described by the principal-component analysis.

(G) Expression profile of angiotensin-converting enzyme 2 (normalized spectral counts) and an OTU from the Bacteroidales (normalized OTU read counts) throughout the course of the experiment (mean ± SEM).

See also Figures S4 and S5 and Tables S5, S6, and S7.